AFLP: a new technique for DNA fingerprinting
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چکیده
منابع مشابه
A PCR-based DNA fingerprinting technique: AFLP for molecular typing of bacteria.
Amplified restriction fragment polymorphism (AFLP) is a PCR-based DNA fingerprinting technique. In AFLP analysis, bacterial genomic DNA is digested with restriction enzymes, ligated to adapters, and a subset of DNA fragments are amplified using primers containing 16 adapter defined sequences with one additional arbitrary nucleotide. Polymorphisms of different Escherichia coli strains or Agrobac...
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Amplified fragment length polymorphisms (AFLPs) are polymerase chain reaction (PCR)-based markers for the rapid screening of genetic diversity. AFLP methods rapidly generate hundreds of highly replicable markers from DNA of any organism; thus, they allow high-resolution genotyping of fingerprinting quality. The time and cost efficiency, replicability and resolution of AFLPs are superior or equa...
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A new type of fingerprinting technique is presented, based on amplified fragment length polymorphism (AFLP). Rather than two endonucleases as in AFLP, we propose the use of three enzymes, hence the method is called three endonuclease (TE)-AFLP. Genomic DNA is digested and two sets of adapters are selectively ligated onto the restriction fragments in a single reaction volume. No adapters complem...
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M.J. Bibb. 1983. Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J. Gen. Microbiol. 129:22572269. 6.Huang, C.H., Y.L. Yang, Y.S. Lin and C.W. Chen. 1998. The telomeres of the Streptomyces chromosomes contain conserved palindromic sequences with potentials to form complex secondary structures. Mol. Microbiol. 28:905-916. 7.Kinashi, H. and M. Shimaji-Murayama. 1991. Ph...
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In the last fifteen years, three devastating fungal diseases of Theobroma cacao, the chocolate tree, have enveloped virtually all areas of cocoa production in Central and South America. One of the common mechanisms for dealing with plant disease problems, particularly in areas with minimal resources for application of fungicides, has been the use of genetically resistant germplasm lines. Unfort...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1995
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/23.21.4407